If it were a computer simulation, there would be bugs, crashes, glitches, and other imperfections. External intrusions into the perceived reality (Alien UFOs are actually sysadmins running tests, etc.).
One of the most distressing parts of the simulation argument, is that I can't really get away from the idea that if we are in a simulation, the most likely simulation to be in would be some broken dev or QA build.
OK so what if we're in a.. video game simulation and one of the really difficult issues is getting the emergent populations to not notice that they are being simulated, much like our difficulty with doors. There. Back on topic.
If we live in a computer simulation then there will be most likely bugs and edge cases and buffer overflows and all those things. So if we knew for certain that we live in a simulation then a lot of people would start looking for exploits.
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