Of course it does. Be discreet and don't draw attention to yourself in doing so - it's not really something you want to do when you've got the floor - but if you're not comfortable, you're less focused.
I dunno, I'm pretty sure sticking my hand down my pants to adjust my junk is never appropriate in public. I mean, I've done it if I thought I could get away with it, but I'd be pretty mortified if anyone ever let on that they caught me.
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